Open AccessExploiting Fluorescent Reporter Molecules for Process Analytical Technology (PAT)
Author(s) -
Tobias Broger,
Res P. Odermatt,
Pablo Ledergerber,
Bernhard Sonnleitner
Publication year - 2009
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2009.171
Subject(s) - process analytical technology , downstream processing , context (archaeology) , fluorescence , process engineering , process (computing) , downstream (manufacturing) , food and drug administration , green fluorescent protein , biochemical engineering , computer science , nanotechnology , chemistry , engineering , work in process , materials science , chromatography , physics , mathematics , paleontology , operations management , statistics , quantum mechanics , biology , operating system , biochemistry , gene
Process Analytical Technology (PAT) launched by the U.S. Food and Drug Administration (FDA) aims to ensure the quality of pharmaceutical products. One important tool to obtain a deeper process understanding is the use of modern real-time process sensors and analyzers. In this context an industrially accepted optical probe for turbidity measurement (Aquasant probe) has been adapted for on-line and real-time fluorescence measurement in (bio-)reactors. By tagging product proteins with fluorescent reporter molecules (e.g. GFP) it was possible to quantify product concentrations during a fermentation process with the new probe. The on-line obtained signal showed a high correlation to the off-line signal. This new method has remarkable advantages compared to classical off-line product lab analyses and can serve as a platform-independent quantification method of products to be used in either up- or downstream processing.