Open AccessA Simple Method for High-Throughput Extract Prefractionation for Biological Screening
Author(s) -
David R. Appleton,
Antony D. Buss,
Mark S. Butler
Publication year - 2007
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2007.327
Subject(s) - fractionation , high throughput screening , throughput , chemistry , chromatography , computer science , biochemistry , wireless , telecommunications
The screening of crude natural product extracts can be problematic, especially in enzyme/protein-based assays, due to promiscuous inhibitors and interference compounds, while high salt and lipid content can significantly dilute compounds of interest. Described herein is a simple method for high-throughput fractionation of crude extracts that can help address some of these issues. Results obtained indicate a significant improvement in assay hit rates without a reduction of hit quality and show how prefractionation aids the discovery of compounds that would be undetected by screening crude extracts alone.