Open AccessSyntheses of Fluorescent Vitamin B12-Pt(II) Conjugates and their Pt(II) Release in a Spectroelectrochemical Assay
Author(s) -
Pilar RuizSánchez,
Stefan Mundwiler,
Roger Alberto
Publication year - 2007
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.2007.190
Subject(s) - chemistry , fluorescence , imidazole , moiety , platinum , conjugate , adduct , cisplatin , stereochemistry , biochemistry , organic chemistry , biology , catalysis , mathematical analysis , physics , mathematics , quantum mechanics , chemotherapy , genetics
The cisplatin adduct of vitamin B12, cis-[PtCl(NH3)2B12]+ (1) reacts with dansyl-imidazole to form cis-[Pt(dansyl-imidazole)(NH3)2B12] (2). In a spectroelectrochemical assay, Co(III) in 2 was reduced to Co(II) under concomitant release of the Pt(II) complex. Since similar reduction occurs in the intracellular space, 2 can be used for studying cell uptake monitored by fluorescent microscopy. The insertion of a fluorescent marker into the platinum moiety will also facilitate the isolation of the platinum complex released from B12 and therefore to characterize its chemical authenticity.