Open AccessIsolierung, HPLC-Trennung und Quantifizierung der Sesquiterpenfraktion von Petasites hybridus (L.) G. M. et SCH.
Author(s) -
Bertold Debrunner,
Markus Neuenschwander
Publication year - 1994
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/chimia.1994.564
Subject(s) - sesquiterpene , chemistry , high performance liquid chromatography , stereochemistry , chromatography
A simple and easily reproducible HPLC procedure allows to separate total extracts of Petasites hybridus (L.) G. M. et SCH. and to detect numerous sesquiterpenes (in most cases sesquiterpene esters) of the general formula 1 (isopetasols), 2 (neopetasols), 3 (petasols), 4 (13-substituted isopetasols), and 5 (13-substituted neopetasols). Four so far unknown sesquiterpene esters 2f (neo-S-petasin), 2g ((E)-3-(methylthio)-acryloyl-neopetasol), 3g ((E)-3-(methylthio)-acryloyl-petasol), and 5b (3-desoxy-13-(angeloyloxy)-neopetasol) have been isolated and the contents of the pharmacologically most interesting compounds isopetasin (1b), neopetasin (2b), and petasin (3b), have been quantitatively determined. Additionally two of the basic alcohols petasol and isopetasol have been detected for the first time in small amounts in roots of P. hybridus.