Open AccessCovalent Labeling of Fusion Proteins with Chemical Probes in Living Cells
Author(s) -
Susanne Gendreizig,
Antje Keppler,
Alexandre Juillerat,
Thomas Gronemeyer,
Horst Pick,
Horst Vogel,
Kai Johnsson
Publication year - 2003
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/000942903777679433
Subject(s) - covalent bond , cysteine , dna , chemistry , in vivo , biochemistry , fusion protein , alkyl , biology , enzyme , recombinant dna , genetics , gene , organic chemistry
A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl group from O6-alkylguanine-DNA to one of its cysteine residues. Labeling is possible in bacterial as well as eukaryotic cells and is independent of the nature of the label, thereby opening up new ways to study proteins in vivo.