Open AccessVersatile Monitoring Tools in Parallel Solid-Phase Synthesis
Author(s) -
E. Felder,
Katia Martina,
Simona Scarpella,
Marco Tatò
Publication year - 2003
Publication title -
chimia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.387
H-Index - 55
eISSN - 2673-2424
pISSN - 0009-4293
DOI - 10.2533/000942903777679299
Subject(s) - workflow , computer science , combinatorial chemistry , automation , solid phase synthesis , drug discovery , process (computing) , biochemical engineering , process engineering , combinatorial synthesis , lead (geology) , chemistry , nanotechnology , materials science , engineering , programming language , database , peptide , biochemistry , mechanical engineering , geomorphology , geology
Parallel synthesis on solid-phase is routinely applied in our combinatorial chemistry efforts for lead finding and lead optimization in drug discovery. The synthetic products are released in solution, isolated individually, and purified, but most of the synthetic process is carried out on solid-phase. This facilitates lab automation and enhances throughput. The vastly increased synthetic productivity poses the question of how to best characterize the quality of compound libraries in an efficient and accurate way. In order to provide maximal information with minimal sample consumption, we have integrated a combination of analytical techniques in our workflow. The techniques, comprising IR, NMR, LC-MS, and HPLC online nitrogen detection (CLND), are illustrated on simple examples, but are usually applied to hundreds of molecules, and recurrently, within the timeframe of a library production.