Open AccessAmplificación cruzada de microsatélites en dos especies de haemúlidos (Haemulon aurolineatum y Haemulon steindachneri)
Author(s) -
Paula E. Pabón Quintero,
José Tavera,
Ana María Millán-Márquez,
P Arturo Acero
Publication year - 2018
Publication title -
boletin de investigaciones marinas y costeras/boletín de investigaciones marinas y costeras
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.177
H-Index - 14
eISSN - 2590-4671
pISSN - 0122-9761
DOI - 10.25268/bimc.invemar.2018.47.1.743
Subject(s) - population , biological dispersal , biology , microsatellite , genetics , gene , demography , sociology , allele
Genetic-population studies in marine fish have allowed to study patterns of dispersal and connectivity between habitats. Oneimportant tool in population genetics is the use of microsatellite molecular markers. Cross-amplification of microsatellite is amethod that consists in using primers designed for one species in a different one but phylogenetically related. Because of theimportance of genetic studies of populations in artisanal fisheries species, primers were evaluated and designed for the species Haemulon aurolineatum and Haemulon steindachneri. Samples were collected from the artisanal fisheries in Barú-Colombia. Amplification was standardized for 12 microsatellites which ten were polymorphic for H. aurolineatum and nine for H. steindachneri. It is considered that the primers implemented in this study are useful for future studies of gene flow in these species.