Open AccessSELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS AISLADAS A PARTIR DE SEDIMENTOS DEL CARIBE COLOMBIANO
Author(s) -
Silvia Narváez-Flórez,
Martha L. Gómez,
María Mercedes Martínez
Publication year - 2016
Publication title -
boletin de investigaciones marinas y costeras/boletín de investigaciones marinas y costeras
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.177
H-Index - 14
eISSN - 2590-4671
pISSN - 0122-9761
DOI - 10.25268/bimc.invemar.2008.37.1.182
Subject(s) - enterobacter , pseudomonas , bacteria , chemistry , crude oil , microbiology and biotechnology , biology , food science , escherichia coli , biochemistry , geology , petroleum engineering , genetics , gene
Thirty one bacterial isolations in minimal salts supplemented medium with hydrocarbons (ACPM or crude oil) as sole carbon source were isolated from sediment samples from the Colombian Caribbean. Bacterial strains underwent selection tests in different concentrations of hydrocarbons; 11 tolerant crude oil and ACPM strains in a range of 1-8%v/v were chosen. A mixed bacterial culture was created and assessed its ability to degrade hydrocarbons in a laboratory-scale test, with a concentration of 2% v/v of ACPM over a period of 21 days. Measurements of biomass in Colony Forming Units (CFU)/mL were used to develop the growth curve of the mixed culture. Hydrocarbons remotion was measured by mass chromatography. The mixed culture was able to degrade the 68.6% of aliphatic hydrocarbons in preference of long chain n- alkanes (C12- C31), reaching a maximum growth of 3.13 x 109 UFC / mL. Degradation of aromatic hydrocarbons was not evidenced under the observation time. Nine of the eleven strains were identified using the biochemical systems BBL and API 50 CHB/E; they belonged to the genus Klebsiella, Chromobacterium, Flavimonas, Enterobacter, Pseudomonas, and Bacillus. The evaluated strains have enzymatic potential to degrade hydrocarbons and it is necessary to characterize them at molecular level in order to develop and effective consortium for field application.