Open AccessComparative HPTLC Analysis of Leaves of Allium cepa L., Ficus carica L. and Ziziphus mauritiana L. with Standard Quercetin
Author(s) -
Hemant D. Une,
D. Khan,
Magdy M. D. Mohammed
Publication year - 2017
Publication title -
international journal of pharmacognosy and phytochemical research
Language(s) - English
Resource type - Journals
ISSN - 0975-4873
DOI - 10.25258/phyto.v9i5.8157
Subject(s) - carica , quercetin , mauritiana , ziziphus , flavonols , kaempferol , ficus , allium , chemistry , traditional medicine , isorhamnetin , myricetin , vernonia amygdalina , botany , flavonoid , ocimum , food science , antioxidant , biology , biochemistry , medicine
Flavonols aglycones are well established in plant extracts with omnipresence of common flavonols like kaempferol,quercetin and myricetin. Quercetin has been reported for various pharmacological activities with major ones in the field ofinflammation, cancer, arthritis, diabetes and its complications. Leaves of plants selected for this study viz. Allium cepa L.,Ficus carica L. and Ziziphus mauritiana L. reported to have antioxidant, anticancer, hypoglycemic activities. It ishypothetised thisactivities are due to flavonoids present in these plants. Hence the ethylacetate fraction of methanolicextract of these leaves was obtained as it provides flavanoid rich fraction and the samples were prepared and HPTLCanalysis was performed against standard quercetin and their quantitative analysis was performed, the solvent systemtoluene: ethyl acetate: formic acid (9: 1: 0.5) was selected. It was observed that these plants shows presence of quercetinwhere A. cepa has highest concentration with F. carica and Z. mauritiana having quercetin in declining order. Thecalibration curve was linear in the range of 0.1 to 0.7 μg for quercetin. Further a correlation coefficient of 0.999 indicatesgood linearity between concentration and area. Thus, it was proved that the plants used in the study has presence ofquercetin which is possibly the reason having the pharmacological uses.