Open AccessThe Effect of Helichrysum odoratissimum (L.) Sweet on Cancer Cell Proliferation and Cytokine Production
Author(s) -
Danielle Twilley,
N. Kishore,
D. Meyer,
Indres Moodley,
V. Kumar,
Namrita Lall
Publication year - 2017
Publication title -
international journal of pharmacognosy and phytochemical research
Language(s) - English
Resource type - Journals
ISSN - 0975-4873
DOI - 10.25258/phyto.v9i5.8138
Subject(s) - hela , cytotoxicity , cell culture , chemistry , microbiology and biotechnology , apoptosis , a431 cells , epidermoid carcinoma , cytokine , biochemistry , biology , cell , immunology , cell cycle , cancer , in vitro , oncogene , genetics
The cytotoxicity of Helichrysum odoratissimum against human epidermoid carcinoma (A431), malignant melanoma(A375), cervical epithelial carcinoma (HeLa) and human embryonic kidney (HEK-293) cells was investigated. Thecytokine production and antioxidant activity was also determined. H odoratissimum was selected based on its traditionalusage as a dressing for wounds. In this study the cytotoxicity was performed on skin and cervical cancer which areassociated with the presence of wounds. H. odoratissimum showed inhibitory activity against A431 cells, with a fiftypercent inhibitory concentration (IC50) of 15.5±0.2 µg/ml, and a high DPPH inhibition with an IC50 of 5.13±0.07 µg/ml.A431 cells treated with the extract showed an increase in morphological characteristics associated with apoptosis. Theextract induced IL-12 in U937 cells and inhibited IL-8 at the tested concentrations, with the highest levels being 12.4±7pg/ml and 103±6.1 pg/ml respectively. IL-2 was inhibited by 56 % and 52 % in naive and PHA induced murine splenocytesrespectively. Furthermore, 2 lipophilic fractions and 3 compounds were isolated from the extract. The lipophilic fractionsshowed relative cytotoxicity on A431 cells with IC50 values of 175±13.5 µg/ml and 61.3±0.16 µg/ml. The extract showedsignificant hepatoprotection at 25 µg/ml on HepG2 cells exposed to D-Galactosamine. This is the first report of the activityof H. odoratissimum ethanolic leaf stem extract on A431, A375, HeLa and HEK-293 cell lines as well as the isolation oftwo promising lipophilic fractions.