Open AccessSpectrophotometric Determination of Azithromycin using Oxidative Coupling Reaction
Author(s) -
Maadh T. Abdulrahman,
Ihab M. Rashid,
Nashwan H. Ali
Publication year - 2020
Publication title -
international journal of drug delivery technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 9
ISSN - 0975-4415
DOI - 10.25258/ijddt.10.3.15
Subject(s) - chemistry , chromatography , detection limit , oxidative coupling of methane , relative standard deviation , absorption (acoustics) , analytical chemistry (journal) , materials science , organic chemistry , methane , composite material
For determining the azithromycin (AZT) in medicinal and pure formulas, a simple spectrophotometric technique was developed. An approach suggested is dependent on an AZT’s oxidative coupling reaction by sodium period (SPI) and 4-amino antipyrine (AAP) producing a pink colored compound with optimum absorption of 480 nm. Different experimental parameters are extensively researched and mastered, which affects the stability of a colored product formed, then developed. The law of the Beer is obeyed over its concentration range 3 to 44 ppm, whereas the limit of detection and quantification is 0.1908 and 0.5726 ppm, respectively, for a connection factor (r) = 0.9998. Also calculated are the molar absorption of 8.23 × 103 L/mol.cm, and the sensitivity index for Sandell is 7 × 10-5 mg/cm2. A method’s accuracy and precision are tested by also determining a relative standard deviation (RSD) less than 0.645 percent, and 100.189 percent average recovery. Practically possible external interferences about a calculation for AZT are checked in drug tablets. The results demonstrated that the procedure for determining AZT was successful in its application in pharmaceutical preparations. Comparing the literature survey that shows good sensitivity and selectivity, the reliability of the proposed method is chalked in.