Open AccessDetermination of Metochloropramide Hydrochloride by Spectrophotometric Method by using Diazotized p-nitro aniline reagent
Author(s) -
Intidhar D. Sulaiman,
Walaa A. Abd Alrada
Publication year - 2020
Publication title -
international journal of drug delivery technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.133
H-Index - 9
ISSN - 0975-4415
DOI - 10.25258/ijddt.10.1.11
Subject(s) - molar absorptivity , reagent , chemistry , aniline , detection limit , nitro , hydrochloride , chromatography , azo coupling , spectrophotometry , nuclear chemistry , organic chemistry , physics , alkyl , optics
New simple, sensitive, accurate, and inexpensive spectrophotometric technique has been developed for the estimation of Metochloropramide hydrochloride [MCP-HCl] in pure and pharmaceutical preparation. This technique is based on the diazotization of a primary amino group of Metochloropramide hydrochloride [MCP-HCl] with NaNo2 and HCl followed by coupling with p-nitro aniline in alkaline medium to obtain a stable red-colored water-soluble azo-dye, show a maximum absorption (λmax) at 513. 50nm. Bear,s low is obeyed in the concentration range of (0.2–25) μg.ml-1 with molar absorptivity of 2.313×103L.mol-1.cm-1and sandall’s sensitivity 0.0145 μg.mL-2. The limit of detection (LOD) and limit of quantitative were 0.182 μg.mL-1and 0.553 μg.mL-1, respectively. The proposed technique successfully applied to (tablets, syrup, and injection).