Open AccessTHE CERTIFICATION OF SUNFLOWER LINES FROM THE COLLECTION OF THE DON EXPERIMENTAL STATION OF V.S. PUSTOVOIT ALL-RUSSIAN RESEARCH INSTITUTE OF OIL CROPS BY USING DNA MARKERS
Author(s) -
А.V. Golovatskaya,
S.Z. Guchetl
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.25230/conf11-2021-39-43
Subject(s) - allele , microsatellite , locus (genetics) , genotyping , sunflower , genetics , biology , germplasm , genotype , horticulture , gene
The aim of this research was to develop molecular genetic passports of sunflower lines from the collection of the Don experimental station of V.S. Pustovoit All-Russian Research Institute of Oil Crops based on polymorphic fractions of microsatellite DNA. We used 17 lines as a research material. We used 12 pairs of primers for genotyping. We found that the ORS 559 locus was monomorphic for these samples. The rest of the loci had from 2 to 4 alleles. The average number of alleles per locus was 2.75, PIC – 0.49, the effective number of alleles – 2.16. The analysis of the DNA profiles of the lines showed the individuality of the allelic composition of each of them. The analysis of the genetic relations between the lines showed that the studied lines were divided into two groups, with a genetic distance between them of 5.9.