Open AccessTHE TESTING OF SSR MARKERS OF GENES CONTROLLING RESISTANCE TO P. HALSTEDII AND THE SELECTION OF OPTIMAL CONDITIONS FOR PCR
Author(s) -
E.V. Badyanov,
С.А. Рамазанова
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.25230/conf11-2021-19-24
Subject(s) - downy mildew , biology , genotyping , helianthus annuus , genetics , selection (genetic algorithm) , gene , marker assisted selection , sunflower , hybrid , genetic marker , allele , dna , microsatellite , genotype , botany , horticulture , computer science , artificial intelligence
Downy mildew is one of the most harmful diseases of sunflower. The most effective measure of this disease control is the development of resistant varieties and hybrids. The use of molecular markers, in particular DNA markers, allows to control the presence of dominant resistance genes at each stage of breeding. We carried out the selection of the optimal method for the isolation of sunflower DNA and the selection of the optimal temperature regimes of amplification for 10 pairs of primers developed to mark the Pl6, Pl8, Pl13, and PlArg genes. Preliminary, we identified 13 allelic variants that are suitable for DNA genotyping of Helianthus annuus by these loci.