Open AccessTHE APPLICATION OF THE PCR METHOD FOR IDENTIFICATION OF THE BACTERIAL PEA BLIGHT PATHOGEN PSEUDOMONAS SYRINGAE PV. PISI IN SEED AND PLANT MATERIAL OF LEGUMINOUS CROPS
Author(s) -
I.M. Ignatieva,
Olga Y. Slovareva
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.25230/conf11-2021-182-186
Subject(s) - pseudomonas syringae , sativum , bacterial blight , blight , pisum , biology , pathogen , pseudomonas , lepidium sativum , crop , botany , horticulture , microbiology and biotechnology , bacteria , agronomy , gene , germination , biochemistry , genetics
The pathogen of bacterial pea blight Pseudomonas syringae pv. pisi is the main phytopathogen of the edible pea (Pisum sativum L.). An increase in the production of leguminous crops in the Russian Federation and throughout the world requires reliable methods of confirmation of the absence of bacteriosis pathogen. The aim of this work is to study and apply the PCR method in the diagnosis of the bacterial pea blight pathogen using commercial sets, officially approved in the Russian Federation. Together with the PCR methods, these commercial sets showed their efficiency. After complete proof tests, the proposed PCR method can be used as one of the official tests for the detection of the bacterial pea blight P. syringae pv. Pisi in both plant and seed material.