Open AccessTHE BIOLOGICAL PECULIARITIES OF PRODUCER STRAIN OF MICROBIOPREPARATION 11-1 BACILLUS SP. – THE ANATAGONIST OF SUNFLOWER PHOMA ROT PATHOGEN
Author(s) -
Evgeniya Efimtseva
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.25230/conf11-2021-166-171
Subject(s) - nutrient agar , agar , sunflower , sucrose , strain (injury) , agar plate , nutrient , carbon source , food science , incubation , pathogen , biology , liquid medium , chemistry , microbiology and biotechnology , botany , horticulture , bacteria , biochemistry , ecology , genetics , anatomy , chromatography
We studied the cultural and physiological characteristics of the producer strain 11-1 Bacillus sp. – the antagonist of sunflower Phoma rot pathogen to develop the technological regulations for the production of a microbiological preparation in a «wettable powder» form. We studied the cultural characteristics of the producer strain on three agar media: potato sucrose agar (PSA), Czapek’s agar and Tylon-3; the characteristics varied significantly depending on the nutrient medium. The maximum diameter of colonies on the tenth day of incubation developed on the Tylon-3 medium – 66×99 mm. We established the optimal conditions for the cultivation of the strain on liquid nutrient media: temperature – 30–35 °С, medium pH from 6 to 10. We found that molasses is an optimal source of carbon nutrition, while peptone is the most favorable source of nitrogen nutrition. We established that the Tylon-3 medium is the optimal complex liquid nutrient medium for the cultivation of the bacterial strain 11-1 Bacillus sp.