Open AccessBiological features and conditions of the surface cultivation of a strain-producer of microbiopreparation 11-3 Bacillus sp. – an antagonist of fusarium pathogens infecting oil flax
Author(s) -
Л В Маслиенко,
A.Kh. Voronkova
Publication year - 2020
Publication title -
masličnye kulʹtury
Language(s) - English
Resource type - Journals
eISSN - 2412-6098
pISSN - 2412-608X
DOI - 10.25230/2412-608x-2020-4-184-57-62
Subject(s) - wettable powder , strain (injury) , nutrient , corn steep liquor , liquid medium , fusarium , carbon source , bacillus (shape) , food science , biology , botany , chemistry , agronomy , microbiology and biotechnology , fermentation , pesticide , anatomy , chromatography , ecology , biochemistry
To develop a technological regimen of production of microbiopreparation in a preparative form ‘wettable powder’ (WP) we studied biological features and conditions of surface cultivation of the strainproducer 11-3 Bacillus sp. – an antagonist of fusariosis pathogen infecting oil flax. Cultural qualities of the strain-producer were studied in three agarized mediums. The optimal conditions for cultivation of the strain-producer on liquid nutrient mediums are estimated: temperature – 30–35 оС, рН – 8–10. As a source of carbon nutrition, glycerin and molasses can be used. Yeastrel and corn-steep concentrate are the optimal sources of nitrogen nutrition; the Tylon’s medium is optimal compound liquid nutrient medium for cultivation of the perspective bacterial strain 11-3 Bacillus sp. Optimal period of the surface cultivation of the bacterial strain 11-3 Bacillus sp. on the liquid nutrient Tylon’s medium was ten days, and a volume of sowing culture to nutrient medium – 2.0%.