Open AccessBiological features and conditions of surface cultivating of a strain-producer of microbiopreparation Т-1 Trichoderma sp. against pathogen causing fusarium on oil flax
Author(s) -
Л В Маслиенко,
A.Kh. Voronkova
Publication year - 2020
Publication title -
masličnye kulʹtury
Language(s) - English
Resource type - Journals
eISSN - 2412-6098
pISSN - 2412-608X
DOI - 10.25230/2412-608x-2020-3-183-121-128
Subject(s) - mycelium , spore , fusarium oxysporum , horticulture , agar , liquid medium , botany , fusarium , agar plate , nutrient , fungus , biology , trichoderma , food science , chemistry , bacteria , ecology , genetics , chromatography
To develop technological regimen for production of microbiopreparations in a preparation form ‘wetting powder’ we studied biological features and conditions of surface cultivating of a strain-producer Т-1 Trichoderma sp. – an antagonist of pathogen Fusarium oxysporum Schlecht. emend. Shyd. et Hans. var. orthoceras (App. еt Wr.) Bilai and Fusarium poae (Peck) Wollenw., Lewis on oil flax. To study cultural and physiological qualities of the strainproducer we used agar and liquid mediums. Surface cultivation of a fungus on agar and liquid Rudakov’s medium at a temperature 25–30 оС was the most favorable for mycelium growth and spore formation. Stationary fungus cultivation on liquid medium with рН from 3 to 6 provided maximal mycelium growth with spore formation and the highest dry mass. Addition of starch into the Chapek’s nutrient medium caused maximal growth of fungus mycelium and increase of its dry mass. The best source of nitrogen for a fungus strain was corn extract. Rudakov’s and No1 mediums are optimal compound liquid nutrient mediums for a surface cultivation of the strain-producer. Optimal period of the surface cultivation of the fungal strain Т-1 Trichoderma sp. on liquid Rudakov’s nutrient medium was 10 days, and a volume of sowing culture to a nutrient medium – 2.0%.