Open AccessThe primary screening of bacterial strains antagonists from a collection of the biological methods laboratory in VNIIMK to a Phoma rot on sunflower. Part II
Author(s) -
Л В Маслиенко,
A.Kh. Voronkova,
L.A. Datsenko,
Evgeniya Efimtseva,
D.А. Punogina,
S.А. Gaydukova,
V.V. Kazakova,
S.R. Kovalyova
Publication year - 2020
Publication title -
masličnye kulʹtury
Language(s) - English
Resource type - Journals
eISSN - 2412-6098
pISSN - 2412-608X
DOI - 10.25230/2412-608x-2020-3-183-107-113
Subject(s) - phoma , antibiosis , pseudomonas , biology , microbiology and biotechnology , nutrient agar , pathogen , agar , horticulture , bacteria , genetics
There are presented data of the primary screening of bacterial strains antagonists from a collection of the biological methods lab of the V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) to an aggressive isolate of the Phoma rot pathogen (Phoma macdonaldii Boerema) affecting sunflower by a method of double or crops. We estimated 27 bacterial strains of Bacillus genus on the three nutrient mediums (potato-sucrose agar (PSA), Taylon-3 and oat agar (OA). We stated 21 of them possessing antagonistic activity with a type of action mode against Phoma pathogen on sunflower named antibiosis. We determined for three strains presence of a competition for nutrient area without formation of a sterile zone with pathogen. Maximal sterile zone to Phoma rot pathogen was formed by strains: 11-3 Bacillus sp. (21.0 mm) on PSA; B-4 B. circulans (19.0 mm) on a Taylon-3 medium and D 1-1 Bacillus sp. (17.0 mm) on OA. Eight from the nine collection bacterial strains of Pseudomonas genus demonstrated antagonistic activity to Phoma rot on PSA and King’s B, and seven strains – on OA with one type of action mode – antibiosis. Maximal sterile zone to Phoma rot pathogen was formed by strains: Oif 2-1 Pseudomonas sp., 16-2 Pseudomonas sp. and 12-2 Pseudomonas sp. (12.5– 12.0 mm) – on PSA; 16-2 Pseudomonas sp. (16.5 mm) – on King’s B; Oif 2-1 Pseudomonas sp. (18.0 mm) – on ОА.