Open AccessAutomated assessment of spinal fluid examination results in passivehemagglutination reaction in neurosyphilis diagnostics
Author(s) -
Г Л Катунин,
Н В Фриго,
С В Ротанов,
Н. В. Китаева,
В. Г. Нестеренко,
A P Suslov,
Г Л Катунин,
Н В Фриго,
С В Ротанов,
N V Kitayeva,
В. Г. Нестеренко,
A P Suslov
Publication year - 2010
Publication title -
vestnik dermatologii i venerologii
Language(s) - English
Resource type - Journals
eISSN - 2313-6294
pISSN - 0042-4609
DOI - 10.25208/vdv809
Subject(s) - neurosyphilis , medicine , syphilis , complement fixation test , point of care testing , serology , pathology , surgery , immunology , antibody , human immunodeficiency virus (hiv)
Spinal fluid samples collected from 50 patients suffering from neurosyphilis (key group) and 30 patients with a nervous systempathology and craniocerebral injuries without any signs of syphilis in the anamnesis (control group) were examined in the course of regulated serological tests to reveal anti-T. pallidum antibodies (complement-fixation test with a treponemal antigen, complementfixationtest with a cardiolipin antigen, immunofluorescence test with whole spinal fluid, ELISA) as well as in the course of TPHAwith the use of modified methods with the end-point dilution of spinal fluid as 1:4. Visual assessment was used to assess the TPHAresults with the determination of the result positivity degree expressed in conditional units - «pluses», along with the automatedassessment using the Criterion 2 hardware system.Comparative results of visual and instrumental assessments of the spinal fluid study results in TPHA revealed full compliance of the qualitative study results and 97.5% correspondence in the results expressed in conditional units («pluses»), which providessufficient grounds for recommending the Criterion 2 automatic diagnostics complex for TPHA image assessment to be used in clinical and diagnostic laboratories in health care institutions for determining treponemal specific antibodies in spinal fluid for neurosyphilis diagnostics.