Open AccessThe role of T-regulatory cells in the pathogenesis of psoriasis
Author(s) -
V. Khayrutdinov,
A F Mikhaylichenko,
M S Mukhina,
А. В. Самцов,
Evgeny N. Imyanitov,
А. М. Иванов,
В. Р. Хайрутдинов,
A F MICHAILICHENCO,
M A MUKHINA,
А. В. Самцов,
Evgeny N. Imyanitov,
А. М. Иванов
Publication year - 2011
Publication title -
vestnik dermatologii i venerologii
Language(s) - English
Resource type - Journals
eISSN - 2313-6294
pISSN - 0042-4609
DOI - 10.25208/vdv1068
Subject(s) - psoriasis , foxp3 , pathogenesis , flow cytometry , immunology , peripheral blood mononuclear cell , regulatory t cell , medicine , peripheral blood , immunohistochemistry , immune system , monoclonal antibody , antibody , il 2 receptor , biology , t cell , biochemistry , in vitro
T-regulatory cells control the strength and duration of immune response and play an important role in the pathogenesis ofpsoriasis. The goal of the study was to examine the content of T-regulatory cells in peripheral blood and affected skin ofpsoriasis patients, the level of FOXP3 mRNA expression in psoriatic skin. Determination of subpopulations of T-regulatorylymphocytes in peripheral blood was performed using flow cytometry. The median level of FOXP3 mRNA expression wasdetermined in biopsies of the skin by PCR in real time. Immunohistochemistry was performed with monoclonal anti-FOXP3 antibodies. Revealed an increase in the relative content of T-regulatory cells in the peripheral blood of patients withpsoriasis in the progressive period, compared with healthy people. The level of FOXP3 mRNA expression in psoriatic fociwas 3.1 times higher than in healthy individuals. Was noted a 10-fold increase in the number of T-regulatory lymphocytesin the skin of psoriasis patients in the progressive period, compared with skin from healthy donors.