Open AccessUsing the Line Blot Syphilis test system for diagnosingsyphilis by the linear immunoblotting method
Author(s) -
C. Г. Марданлы,
В. А. Арсеньева,
Н В Фриго,
С В Ротанов,
Е. А. Амелина,
М. В. Захаров,
С. Г. Марданлы,
V A Arseniyeva,
Н В Фриго,
С В Ротанов,
Ye A Amelina,
М. В. Захаров
Publication year - 2011
Publication title -
vestnik dermatologii i venerologii
Language(s) - English
Resource type - Journals
eISSN - 2313-6294
pISSN - 0042-4609
DOI - 10.25208/vdv1022
Subject(s) - syphilis , western blot , immunofluorescence , immunoassay , virology , antibody , medicine , microbiology and biotechnology , chemistry , immunology , biology , biochemistry , human immunodeficiency virus (hiv) , gene
ZAO ECOlab (Russia) developed Line Blot Syphilis, a new test system on the basis of the linear immunoblotting methodusing Treponema рallidum recombinant antigens. The article assessed the diagnostic value of the Line Blot Syphilis testsystem in the form of a confirmatory test. As a part of the conducted study, the test system demonstrated its absolutesensitivity and specificity to serum-positive (n = 237) and serum-negative (n = 114) samples, in which the presenceor absence of Т.pallidum antibodies was confirmed by two treponemal tests. As a result of examining 14 samplesattributed to doubtful analytes based on two test results (passive hemagglutination test and immunofluorescence testwith absorption), the data compliance between the Line Blot Syphilis test system and data from INNO-LIA Syphilis Scoreamounted to 100% (14/14) or 93% (13/14) for the immune-enzyme assay and 57% (8/14) for the immunofluorescencetest with absorption. It is recommended to use the Line Blot Syphilis test system (ZAO ECOlab) as a confirmatory syphilistest.