Open AccessThe role of molecular and genetic methods to determine theТ-cell and В-cell clonality in diagnosing malignant cutaneouslymphomas
Author(s) -
V A Molochkov,
Yu S Sidorova,
A A Groznova,
G V Ovsyannikova,
A V Fedorovskaya,
A V Molochkov,
Andrey Sudarikov,
A V Molochkov,
Yu S Sidorova,
A A Groznova,
G V Ovsyannikova,
A V Fedorovskaya,
A V Molochkov,
Andrey Sudarikov
Publication year - 2011
Publication title -
vestnik dermatologii i venerologii
Language(s) - English
Resource type - Journals
eISSN - 2313-6294
pISSN - 0042-4609
DOI - 10.25208/vdv1018
Subject(s) - pseudolymphoma , mycosis fungoides , polymerase chain reaction , pathology , cell , cutaneous lymphoma , polyclonal antibodies , lymphoproliferative disorders , b cell , medicine , lymphoma , gene rearrangement , differential diagnosis , antibody , gene , dermatology , biology , immunology , genetics
The article presents the data on the studies contributing to improving the differential diagnostics of T-cell and B-cellcutaneous lymphomas including large-plaque parapsoriasis and T-cell and B-cell cutaneous pseudolymphomas as wellas frequency of their transformation into malignant cutaneous lymphomas. There was a study of 101 patients using thepolymerase chain reaction method to determine the T-cell and B-cell lymphocyte clonality by genes of g and chains inthe T-cell receptor and immunoglobulin heavy chain genes. Monoclonality was determined in 40 of 46 cases in patientswith T-cell cutaneous lymphomas and in three of four cases in patients with B-cell cutaneous lymphomas. Monoclonalitywas revealed in one of 14 cases of large-plaque parapsoriasis and one of two cases of T-cell cutaneous pseudolymphoma.In all of the 24 cases of chronic benign dermatoses, five cases of small-plaque parapsoriasis and ten skin tissue samplesobtained from healthy donors, polyclonal lymphocytes were revealed. So, the obtained results make it possible toconsider the method to be an important addition in the field of diagnosing lymphoproliferative skin diseases.