Open AccessDermal response to combined double filler administration
Author(s) -
Г. М. Могильная,
E.V. Fomicheva
Publication year - 2020
Publication title -
kubanskij naučnyj medicinskij vestnik
Language(s) - English
Resource type - Journals
eISSN - 2541-9544
pISSN - 1608-6228
DOI - 10.25207/1608-6228-2020-27-4-72-81
Subject(s) - hyaluronic acid , haematoxylin , extracellular matrix , medicine , masson's trichrome stain , vimentin , calcium , hyaluronidase , eosin , pathology , fibrosis , chemistry , staining , anatomy , biochemistry , immunohistochemistry , enzyme
Aim . This experimental work presents a comparative assessment of the effect of hyaluronic acid and crystalline calcium hydroxyapatite on derma in separate and combined application setting. Materials and methods . The study used rats (30 animals) for subdermal injection of 0.05 ml medium. Group 1 was administered calcium hydroxyapatite (Radiesse (Merz, Germany)), group 2 received hyaluronic acid (Restylane (SabQ, Sweden)) and Group 3 — both preparations combined in ratio 1:1 (Mix). Results were evaluated 4 months after the ller injection. Sections were stained with haematoxylin–eosin, van Gieson’s and Masson’s trichrome techniques. Collagen types I and III were detected with polyclonal antibodies (Abcam, England). Fibroblasts were positively identied with vimentin (LabVision), macrophages — with CD68 (LabVision) tagging. Effect of extracellular matrix remodelling was studied with α-SMA actin (Abcam, England). Results . We demonstrate that separate lling of hyaluronic acid and calcium hydroxyapatite produces different response mechanisms, while their combined administration does not lead to a marked voluming of the dermal extracellular matrix. Conclusion . Combined administration of the two llers under study may facilitate a prolonged effect of their combined action exceeding 4 months, due to the absence of brosis, complications and side effects.