Open AccessExperimental Evaluation of Cryoprotector Systemic Effects
Author(s) -
А. К. Мартусевич,
А. А. Костяев,
Lida K. Kovaleva
Publication year - 2019
Publication title -
kubanskij naučnyj medicinskij vestnik
Language(s) - English
Resource type - Journals
eISSN - 2541-9544
pISSN - 1608-6228
DOI - 10.25207/1608-6228-2019-26-6-117-126
Subject(s) - intraperitoneal injection , dimethylacetamide , dimethyl sulfoxide , cryoprotectant , chemistry , pharmacology , systemic administration , medicine , biochemistry , cryopreservation , biology , organic chemistry , in vivo , solvent , embryo , microbiology and biotechnology
Aim . Experimental evaluation of the effect of some cryoprotectors on crystallogenic properties of mice blood serum at intraperitoneal administration. Material and methods . The experiment was performed on 40 adult white line mice divided into 4 equal groups. Animals from the control (fi rst) group received a single intraperitoneal injection of saline (0.5 ml agent/animal), animals of the second-fourth groups — 3 % solution of glycerin, dimethyl sulphoxide and trombocryodmac (N, N-dimethylacetamide), respectively, in the same quantity. Results . It is shown that single intraperitoneal introduction of cryoprotectants (solutions of glycerin, dimethyl sulphoxide and trombocryodmac) has a systemic effect on the mouse organism, which is manifested through the changes of crystallogenic properties of blood serum. 3% solution of glycerin, dimethyl sulphoxide and trombocryodmac have multidirectional infl uence on crystallogenic properties of mouse blood serum. The most pronounced physiological effect was established for dimethyl sulphoxide. Conclusion . Our studies demonstrate the presence of systemic effects in cryopreservative substances. It was confi rmed by the changes of crystallogenic properties of mice blood serum under intraperitoneal administration of cryopreservatives.