Open AccessAnticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS
Author(s) -
Hatice Kızıltaş,
Ahmet Gören,
Zeynebe Bingöl,
Saleh Alwasel,
İlhami Gülçın
Publication year - 2021
Publication title -
records of natural produc
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.43
H-Index - 31
ISSN - 1307-6167
DOI - 10.25135/rnp.236.21.02.1983
Subject(s) - chemistry , antioxidant , dpph , abts , polyphenol , ascorbic acid , chromatography , food science , biochemistry
To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.