Open AccessКОМБІНОВАНА ДІЯ НИЗЬКОЇ КОНЦЕНТРАЦІЇ РАУНДАПУ НА ДВОСТУЛКОВОГО МОЛЮСКА АКТИВУЄ ТІОЛОВІ СПОЛУКИ У ТРАВНІЙ ЗАЛОЗІ
Author(s) -
Vira Khoma,
Lesya Gnatyshyna,
Viktoria Martinyuk,
Tetiana Matskiv,
Kateryna Yunko,
R. Formanchuk,
V. S. Baranovskii,
M. M. Gladyuk,
Levonas Manusadžianas,
Оksana Stoliar
Publication year - 2020
Publication title -
naukovì zapiski. serìâ bìologìâ
Language(s) - English
Resource type - Journals
ISSN - 2078-2357
DOI - 10.25128/2078-2357.20.3-4.9
Subject(s) - oxidative stress , lipid peroxidation , catalase , glutathione , superoxide dismutase , glyphosate , chemistry , metallothionein , antioxidant , toxicology , toxicity , xenobiotic , environmental chemistry , zoology , biochemistry , biology , enzyme , ecology , organic chemistry , gene
Glyphosate is one of most popular weed killers in the word. Its toxicity to aquatic organisms was investigated mostly in the acute high experimental exposures. The aim of this study was to evaluate the effect of low, 0.5 of Predicted No Effect Concentration (PNEC), of glyphosate in the combinations to freshwater bivalve mollusks.We treated the mussels Unio tumidus with glyphosatebased herbicide RoundupMAX (Rn, correspondent to 16.9 µg L-1 or 40 nM of glyphosate) in the combination with heating 25o C (RnT) or chlorpromazine (RnCpz, 18.0 µg L-1 or 56 nM of Cpz) and Cpz alone during 14 days. The responses of oxidative stress were evaluated in the digestive gland. The enzyme activities were changed only by the exposures to Rn (increase of superoxide dismutase) and Cpz (decrease of catalase), whereas the elevation of total glutathione (GSH) level was indicated in all exposures except Rn, and metallothonein-associated thiols (MTSH) – in all exposures except Cpz. Lipid peroxidation was increased in all exposures by 16.6% maximally. Total balance of antioxidants versus prooxidative changes was increased in all exposures contained Rn (by ~3 times in RnT-group) and decreased in the exposure to Cpz alone. Metallothionein chromatographyc features did not indicate substantial oxidative changes in all exposed groups. Hence, combine exposures distort prominently the oxidative stress responses to xenobiotics in the freshwater mussels even in low, nanomolar concentrations. The ability of Rn to induce MTSH seems to be the decisive input in the antioxidant defence at combine exposures.