Open AccessВМІСТ ФОТОСИНТЕТИЧНИХ ПІГМЕНТІВ У РОСЛИНАХ РОДУ CARLINA L. У ПРИРОДІ ТА КУЛЬТУРІ IN VITRO
Author(s) -
Н. Б. Кравец,
L. R. Hrytsak,
M. Z. Prokopyak,
О. Yu. Маyorova,
Н. М. Дробык
Publication year - 2020
Publication title -
naukovì zapiski. serìâ bìologìâ
Language(s) - English
Resource type - Journals
ISSN - 2078-2357
DOI - 10.25128/2078-2357.19.4.3
Subject(s) - gibberellic acid , sterilization (economics) , horticulture , aseptic processing , petri dish , botany , biology , germination , food science , genetics , monetary economics , economics , foreign exchange market , foreign exchange
im. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treatment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentrations of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators.