Open AccessRapid diagnostic methods for SARS-CoV-2 (COVID-19) detection: an evidence-based report
Author(s) -
Manish Verma,
Parshant Kumar Sharma,
Henu Kumar Verma,
Anand Narayan Singh,
Desh Deepak Singh,
Pratima Verma,
Areena Hoda Siddiqui
Publication year - 2021
Publication title -
journal of medicine and life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.354
H-Index - 36
eISSN - 1844-3117
pISSN - 1844-122X
DOI - 10.25122/jml-2021-0168
Subject(s) - covid-19 , medicine , pandemic , molecular diagnostics , coronavirus , transmission (telecommunications) , intensive care medicine , virology , computer science , bioinformatics , disease , infectious disease (medical specialty) , pathology , biology , telecommunications
Since December 2019, the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been a global health concern. The transmission method is human-to-human. Since this second wave of SARS-CoV-2 is more aggressive than the first wave, rapid testing is warranted to use practical diagnostics to break the transfer chain. Currently, various techniques are used to diagnose SARS-CoV-2 infection, each with its own set of advantages and disadvantages. A full review of online databases such as PubMed, EMBASE, Web of Science, and Google Scholar was analyzed to identify relevant articles focusing on SARS-CoV-2 and diagnosis and therapeutics. The most recent article search was on May 10, 2021. We summarize promising methods for detecting the novel Coronavirus using sensor-based diagnostic technologies that are sensitive, cost-effective, and simple to use at the point of care. This includes loop-mediated isothermal amplification and several laboratory protocols for confirming suspected 2019-nCoV cases, as well as studies with non-commercial laboratory protocols based on real-time reverse transcription-polymerase chain reaction and a field-effect transistor-based bio-sensing device. We discuss a potential discovery that could lead to the mass and targeted SARS-CoV-2 detection needed to manage the COVID-19 pandemic through infection succession and timely therapy.