Open AccessIdentification of messenger RNA of fetoplacental source in maternal plasma of women with normal pregnancies and pregnancies with intrauterine growth restriction
Author(s) -
Paola Ayala-Ramírez,
Reggie García-Robles,
Juan Diego Rojas Barrera,
Martha Bermúdez,
Jaime Eduardo Bernal Villegas
Publication year - 2012
Publication title -
colombia medica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.455
H-Index - 18
eISSN - 1657-9534
pISSN - 0120-8322
DOI - 10.25100/cm.v43i3.863
Subject(s) - intrauterine growth restriction , rna , placenta , human placental lactogen , messenger rna , pregnancy , placental lactogen , endocrinology , gene expression , gestation , medicine , biology , andrology , gene , fetus , genetics
Objective: to quantify placenta-specific RNA in plasma of women carrying foetuses with intrauterine growth restriction andpregnant women with normal pregnancies.Materials and methods: 8 pregnant women with foetuses with intrauterine growth restriction were studied as well as 18women with uncomplicated pregnancies in the third pregnancy trimester. Total free RNA was quantified in maternal plasmaby spectrophotometry and the gene expression of hPL (Human Placental Lactogen) at the messenger RNA level throughtechnical Real Time-Chain Reaction Polymerase.Results: plasma RNA of fetoplacental origin was successfully detected in 100% of pregnant women. There were no statisticallysignificant differences between the values of total RNA extracted from plasma (p = 0.5975) nor in the messenger RNAexpression of hPL gene (p = 0.5785) between cases and controls.Conclusion: messenger RNA of fetoplacental origin can be detected in maternal plasma during pregnancy.