Open AccessCharacterization of root-associated bacterial species of Leklek variety of common bean (Phaseolus sp.)
Author(s) -
Dilek Tekdal,
İlknur Akça,
Aslı Küçükrecep,
Selim Çetiner,
Rüştü Hatipoğlu
Publication year - 2021
Publication title -
romanian biotechnological letters
Language(s) - English
Resource type - Journals
eISSN - 2248-3942
pISSN - 1224-5984
DOI - 10.25083/rbl/26.5/3008.3013
Subject(s) - phaseolus , biology , ribosomal rna , 23s ribosomal rna , 16s ribosomal rna , internal transcribed spacer , phylogenetic tree , ribosomal dna , botany , gene , genetics , rna , ribosome
The common bean is a valuable food source in the human diet. Leklek is a local variety of common bean (Phaseolus sp.) widely grown in Mersin's Gülnar district, but little is known about this variety. In the present study, bacterial species from root nodules of this common bean variety were identified by PCR-amplified 16S ribosomal RNA (rRNA) gene and 16S-23S ribosomal RNA (rRNA) Internal Transcribed Spacer (ITS) region and sequencing. The partial 16S rRNA gene and 16S-23S rRNA ITS region sequences were submitted to the NCBI database (accession numbers MT967369, MT968518, respectively). Amplified sequences were used to construct a phylogenetic tree. Phylogenetic analysis based on the identified sequences showed that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium paraoxydans. The findings presented here will provide a clue for understanding this bacterium's role in nodule formation in Phaseolus sp. (variety Leklek).