Open AccessGene Transformation by Chitosan Nanoparticle to Enhance Fatty Acid Production in Zea mays (L.)
Author(s) -
EMAN TAWFIK HUSSIEN,
MOHAMMED IBRAHIM DAHAB,
KAREEM MOHAMMED ABD-ELATTY,
ISLAM HAMDY EL-SHENAWY,
HEBA MAHMOUD FAROUK,
AYA TAREK RIZK,
NADA AHMED KAMAL,
RAWAN ADEL ABD-ELWAHAB
Publication year - 2021
Publication title -
romanian biotechnological letters
Language(s) - English
Resource type - Journals
eISSN - 2248-3942
pISSN - 1224-5984
DOI - 10.25083/rbl/26.5/2971.2978
Subject(s) - transgene , transformation (genetics) , genetically modified maize , biochemistry , fatty acid , acetyl coa carboxylase , chemistry , genetically modified crops , gene , genetically modified rice , biosynthesis , fatty acid synthesis , enzyme , food science , biology , pyruvate carboxylase
Zea mays is an important crop and an essential source of fatty acids. Hence, increasing and adding new fatty acids led to the enhancement of these properties. Transformation of external Acetyl-CoA gene (Aco) can enhance fatty acid components, as ACo is expressed into Acetyl-CoA carboxylase (ACCase) enzyme, which is the first essential step in the fatty acid production process. Chitosan nanoparticles are safe and fast polymer nanoparticles that are applied for gene transformation. Conventional PCR was performed for the detection of the ACo gene in both transgenic and nontransgenic maize lines. The results confirm the presence of the gene in the transgenic lines and absence in non-transgenic lines. The Gas chromatography-mass spectrometry (GC-MS) analysis for fatty acid contents in transgenic and non-transgenic maize lines showed an increase in fatty acid contents in transgenic lines compared to non-transgenic ones. Besides, the transgenic maize’s lines produced extra new fatty acids not found in non-transgenic ones.