Open AccessScreening of local wild-type Xanthomonas spp. for xanthan biosynthesis using media with different carbon sources
Author(s) -
Ida Zahović,
Jelena Dodić,
Siniša Markov,
Jovana Grahovac,
Mila Grahovac,
Zorana Rončević
Publication year - 2021
Publication title -
romanian biotechnological letters
Language(s) - English
Resource type - Journals
eISSN - 2248-3942
pISSN - 1224-5984
DOI - 10.25083/rbl/26.4/2800-2807
Subject(s) - xanthomonas campestris , xanthomonas , food science , glycerol , xanthan gum , pepper , biosynthesis , botany , biology , horticulture , biochemistry , enzyme , materials science , rheology , gene , composite material
In this study the screening of different Xanthomonas strains, isolated from infected crucifers and pepper leaves, for xanthan biosynthesis on semi-synthetic media containing different carbon sources was performed. The success of xanthan biosynthesis was estimated based on xanthan concentration in media and its molecular weight. Glucose and glycerol were investigated as carbon sources in a quantity of 20.0 g/L. Xanthan biosynthesis by different Xanthomonas isolates on two different cultivation media was carried out in Erlenmeyer flasks under aerobic conditions for 168 h. According to the obtained results selection of the carbon source, producing strain and their combination have a statistically significant effect on xanthan quantity and quality. The results obtained in this study indicate that local wild-type Xanthomonas strains isolated from pepper leaves have a great potential for application in biotechnological production of good-quality xanthan on glycerol-based media.