Open AccessEffects of simulated microgravity on the morphology of mouse embryonic fibroblasts (MEFs)
Author(s) -
Hoàng Nghĩa Sơn,
Hồ Nguyễn Quỳnh,
Le Ngoc Phuong Thanh,
Truong Thi Han,
Nguyen Thai Minh Han,
DOAN CHINH CHUNG,
Lê Thanh Long
Publication year - 2020
Publication title -
romanian biotechnological letters
Language(s) - English
Resource type - Journals
eISSN - 2248-3942
pISSN - 1224-5984
DOI - 10.25083/rbl/25.6/2156.2160
Subject(s) - embryonic stem cell , flow cytometry , fibroblast , morphology (biology) , microbiology and biotechnology , biophysics , actin , chemistry , cell , phase contrast microscopy , biology , biochemistry , in vitro , genetics , gene , physics , optics
This study aimed to assess the effects of simulated microgravity on mouse embryonic fibroblast (MEF) morphology. The results showed that the area of MEFs under simulated microgravity was 7843.39 ± 551.31 µm2 which was lower than the control group (9832.72 ± 453.86 µm2). The nuclear area of MEFs under simulated microgravity (290.76 ± 4.58 µm2) and the control group (296.8 ± 4.58 µm2) did not statistically differ. In addition, the nuclear shape value of the MEFs under simulated microgravity and the control group did not statistically differ (0.86 ± 0.006 vs. 0.87 ± 0.003, respectively). The nuclear intensity of MEFs under simulated microgravity (19361 ± 852) was higher than the control group (16997 ± 285). Moreover, the flow cytometry analysis indicated the reduced G0/G1 phase cell ratio and the increased S phase and G2/M phase cell ratio in MEFs under simulated microgravity. Simulated microgravity also induced a decrease in diameter of actin filament bundles of the MEFs under simulated microgravity (1.61 ± 0.33 µm) compared to the control group (1.79 ± 0.32 µm). These results revealed that simulated microgravity is capable of inducing the morphological changes of mouse embryonic fibroblasts.