Open AccessSynergistic effects of Ruta montana (Clus.) L. essential oil and antibiotics against some pathogenic bacteria
Author(s) -
Azzeddine Zeraib,
Lamia Boudjedjou,
Naziha Suici,
Tarek Benmeddour,
K Rahal,
Azzedine Fercha
Publication year - 2021
Publication title -
journal of phytology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.137
H-Index - 2
ISSN - 2075-6240
DOI - 10.25081/jp.2021.v13.7088
Subject(s) - antibiotics , essential oil , pathogenic bacteria , agar dilution method , antibacterial activity , bacteria , agar diffusion test , agar dilution , antibiotic resistance , microbiology and biotechnology , chemistry , biology , food science , traditional medicine , medicine , minimum inhibitory concentration , genetics
Antibiotic resistance has been called one of the world’s most pressing public health threats. The combination of essential oils with conventional antibiotics is one of the emerging approaches that could help prevent this problem. In light of this, this study aimed to investigate the impact of combination of Ruta montana essential oil with conventional antibiotics on some pathogenic bacteria. The essential oil isolated by steam-hydrodistillation was first analyzed using GC-MS then tested alone and in combination with five recommended antibiotics against three bacterial strains by the agar disc diffusion and broth micro-dilution methods. Out of forty-nine peaks, thirty-eight components were identified representing 98.17% of the total oil composition. The major components were 2-Undecanone (63.39%), 2-Nonanone (5.65%), 2-Acetoxytetradecane (4.94%), 2-Decanone (4.47%) and 2-Dodecanone (3.35%). While R. montana essential oil showed only weak antibacterial activity compared to the antibiotics tested alone, unexpectedly, the combination of RM essential oil with antibiotics remarkably increased the antibacterial activity of the antibiotics through synergistic effects in up to 70% of cases. These results suggest that combining antibiotics with essential oils, even those with low antibacterial activity, may be effective in overcoming problems caused by increasing bacterial resistance.