Open AccessPHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF ZILLA SPINOSA EXTRACTS AGAINST PATHOGENIC MICROORGANISMS
Author(s) -
Oday N. Al-hadithy,
Suliman Mohammed Suliman Alghanem,
Mohamed B. Milad
Publication year - 2018
Publication title -
journal of medicinal botany
Language(s) - English
Resource type - Journals
ISSN - 2521-3903
DOI - 10.25081/jmb.2018.v2.987
Subject(s) - phytochemical , antimicrobial , petroleum ether , ethyl acetate , aspergillus niger , biology , traditional medicine , antibacterial activity , bacteria , microbiology and biotechnology , food science , chemistry , botany , biochemistry , extraction (chemistry) , chromatography , medicine , genetics
Zilla spinosa is one of the most common plant species of family Brassicaceae, due to its important uses in the folk medicinal. The present study was aimed to determine some active constituents and to test antimicrobial activity of different plant extracts against pathogenic microorganisms. Fresh plant was collected randomly from northern sector of Eastern Desert, Egypt (Cairo-Suez desert road) in the month of March 2018. Phytochemical analysis of Z. spinosa showed that, it was rich in saponins and tannins (34.68 and 32.82 mg/g dry weight, respectively). While, phenolics attained values (28.22 mg/g dry weight), alkaloids (17.56 mg/g dry weight) and flavonoids (11.22mg/g dry weight). In the present study, in case of petroleum ether extract the inhibition zone varied according to type of examined bacteria. Staphylococcus aureus was the most affected bacteria followed by Stroptococcus pyogenes, Klebsiella pneumonia and Bacillus subtilis. Methylene chloride extract does not affect both K. pneumoniae and Escherichia coli but prevents others. The ethyl acetate extract inhibit S. aureus, S. pyogenes and B. subtilis. The acetone extract inhibited all the bacteria except E. coli and K. pneumonia. Methyl alcohol extract inhibited all bacteria with different inhibition zones except B. subtilis. On the other hand, Methylene chloride extract has effect on Aspergillus fumigatus. The ethyl acetate and methyl alcohol extract inhibited the growth of all tested fungi except A. niger. Acetone inhibited the growth of Mucor spp. and Candidia albicans.