Germplasm conservation of Phyllanthus virgatus G. Forst. by encapsulation of In vitro derived leaf segments

The present study describes an efficient plant regeneration system for in vitro propagation of Phyllanthus virgatus through leaf explant. The leaf explants were cultured on Murashige & Skoog (MS) medium supplemented with BAP (1.5 mg L-1) and NAA (0.9 mg L-1 for efficient callus induction (82.26%). The maximum percentage of shoots (77.85%) was developed from BAP (2.0 mg L-1) and GA3 (0.5 mg L-1). The efficient rooting response (76.28%) was noted on a nutrient medium containing NAA (1.5 mg L-1) and IBA (0.2 mg L-1). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room and then transferred to green house with a 72% survival rate.