Open AccessPemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya
Author(s) -
Cori Yolanda Elise,
Syafrizayanti Syafrizayanti,
Marniati Salim
Publication year - 2021
Publication title -
jurnal riset kimia
Language(s) - English
Resource type - Journals
eISSN - 2476-8960
pISSN - 1978-628X
DOI - 10.25077/jrk.v12i2.412
Subject(s) - phycocyanin , spirulina (dietary supplement) , phycobiliprotein , pigment , chemistry , dpph , allophycocyanin , phycocyanobilin , chromatography , food science , antioxidant , biochemistry , organic chemistry , cyanobacteria , biology , bacteria , raw material , genetics
Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of 338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.