Open AccessImmune Cells Expansion From Peripheral Blood of Patients of Three Leading Cancers in Vietnam
Author(s) -
Chu Thi Bich Thao,
Bùi Việt Ánh,
Nguyễn Văn Phòng,
Nguyen Trung Kien,
Nguyen Dac Tu,
Doan Trung Hiep,
Nguyễn Thanh Liêm,
Hoang Thi My Nhung
Publication year - 2021
Publication title -
tạp chí khoa học đại học quốc gia hà nội: khoa học tự nhiên và công nghệ (vnu journal of science:natural sciences and technology)
Language(s) - English
Resource type - Journals
eISSN - 2615-9317
pISSN - 2588-1140
DOI - 10.25073/2588-1140/vnunst.5291
Subject(s) - immune system , cytotoxic t cell , cd8 , cancer , lung cancer , immunotherapy , liver cancer , cancer immunotherapy , immunology , medicine , cell , cancer research , cancer cell , biology , in vitro , biochemistry , genetics
Immunotherapy recently attracted the high attention of scientists in cancer treatment. However, this therapy is poorly studied and applied in Vietnam. In this report, we present the results of immune cell ex vivo expansion for cancer treatment. After 20-21 days of culture, the average number of CD3+CD8+ cytotoxic T lymphocytes (CTLs) increased by 633.6-fold, equivalent to 3277.5 × 106 cells, and with the cell viability was 96.3%. Among them, the increasing fold of lung, liver and gastric cancer patients were 232.4, 812.4 and 655.6 respectively. Meanwhile, the average number of CD3-CD56+ NK cells increased by 940.3-fold, equivalent to 2659.3 × 106 cells, with the cell viability of 95%. Gastric cancer patients had the highest fold of Natural killer (NK) cell expansion (1644.9 fold) compared to that in lung cancer (695.0 fold) and liver cancer patients (358.3 fold). These results revealed that our method of immune cell expansion met the requirements in the immune cell number for clinical applications in cancer treatment in Vietnam.