Open AccessPaclitaxel - Combined Curcumin - Loaded PLA-TPGS - Based Nanoparticles Induce Apoptosis and Inhibit the Growth of Breast Cancer Cells and Multicellular Tumor Spheroids
Author(s) -
Nguyen Dac Tu,
Tran Phan Anh,
Phuong Thu Ha,
Nguyễn Hoài Nam,
N.X. Phuc,
Hoang Thi My Nhung
Publication year - 2021
Publication title -
tạp chí khoa học đại học quốc gia hà nội: khoa học tự nhiên và công nghệ (vnu journal of science:natural sciences and technology)
Language(s) - English
Resource type - Journals
eISSN - 2615-9317
pISSN - 2588-1140
DOI - 10.25073/2588-1140/vnunst.5284
Subject(s) - curcumin , paclitaxel , cytotoxicity , chemistry , apoptosis , drug delivery , pharmacology , cancer cell , nanoparticle , cancer , nanotechnology , materials science , in vitro , biochemistry , medicine , organic chemistry
Paclitaxel and curcumin have been reported as anti-cancer drugs. Here we presented a novel combination of paclitaxel and curcumin-loaded PLA-TPGS (PTX-Cur/PLA-TPGS) nanoparticles prepared by a modified solvent extraction/evaporation technique. These nanoparticles were well distributed and stable in water. This combination of paclitaxel and curcumin gave a higher efficiency of both drugs in cytotoxicity; induced apoptosis; and effect on cell cycles of KPL4 cell line in compare with the use of paclitaxel or curcumin alone or even a normal mixture of these two drugs. Furthermore, PTX-Cur/PLA-TPGS nanoparticles exhibited a powerful ability in preventing MCF7 spheroids growth. Interestingly, curcumin also functioned as both a drug and a label. Base on the autofluorescence of curcumin, the absorption of PTX-Cur/PLA-TPGS nanoparticles into MCF7 spheroids could be followed and calculated. These results suggested that the nanoparticle-drug combination may provide a promising multifunctional delivery system for anti-cancer drugs.