Open AccessPreparation of agarose-glucan for anti-tumor necrosis factor protein drug delivery
Author(s) -
Hường Pham
Publication year - 2018
Publication title -
tạp chí khoa học đại học quốc gia hà nội: khoa học tự nhiên và công nghệ (vnu journal of science:natural sciences and technology)
Language(s) - English
Resource type - Journals
eISSN - 2615-9317
pISSN - 2588-1140
DOI - 10.25073/2588-1140/vnunst.4758
Subject(s) - agarose , tumor necrosis factor alpha , chemistry , drug delivery , glucan , drug , etanercept , pharmacology , chromatography , biochemistry , immunology , medicine , organic chemistry
Our purpose was to develop and characterize a protein drug delivery system in agarose-glucan complex. The complex was produced by sonicating the mixture of agarose-glucan components and a protein in liquid paraffin with Sonics Vibracell Processor adapted from method of Nuo Wang et all 1997 [1]. We used etanercept, an anti-tumor necrosis factor-alpha (TNF-α) as a model protein drug, which was encapsulated successfully into agarose-glucan complex system. This protein can neutralize the TNF-α, a pro-inflammatory cytokines that plays a pivotal role in regulating the inflammatory response in rheumatoid arthritis (RA) and well known as mediator worsening RA pathogenesis. The agarose-glucan complex we made possessed a range of sizes from 30 to 150 nm, dissolving well within a range of pH buffer from 5.2 to 6.2, an average protein encapsulated efficiency up to 70%, and protein release efficiency of 50% after 24 hours. This research is the base for developing nanogel-size targeted drug delivery in RA treatment.