Open AccessEffects of Carbonyl-cyanide m-chlorophenylhydrazone on Mitochondrial Function of H9C2 Cells
Author(s) -
Ngo Thi Hai Yen,
Bui Khanh,
Vu Thao Hien,
To Thanh Thuy,
Phạm Thị Bích,
Dang Thi Ha Trang,
Vũ Thị Thu
Publication year - 2017
Publication title -
tạp chí khoa học đại học quốc gia hà nội: khoa học y dược (vnu journal of science: medical and pharmaceutical sciences)
Language(s) - English
Resource type - Journals
eISSN - 2615-9309
pISSN - 2588-1132
DOI - 10.25073/2588-1132/vnumps.4095
Subject(s) - acridine orange , cardiolipin , rhodamine 123 , chemistry , inner mitochondrial membrane , mitochondrion , cyanide , membrane potential , rhodamine , biophysics , confocal , fluorescence , membrane , biochemistry , biology , apoptosis , physics , optics , phospholipid , organic chemistry , multiple drug resistance , antibiotics
We examined the effects of carbonyl-cyanide m-chlorophenylhydrazone (CCCP) on mitochondrial function of H9C2 cells. Composition of mitochondrial membrane lipids (cardiolipin) and mitochondrial membrane potential was analyzed by fluorescence intensity change of tetramethl rhodamine ethyl ester (TMRE) and 10-nonyl acridine orange (NAO) using the LSM800 confocal microscope. Our results showed that CCCP strongly and simultaneously affected mitochondrial structure and function of H9C2 cells.