Open AccessSynthesis and Characterization of Silver Nanoparticles for Antibacterial Application against Bacillus Subtilis and Pseudomonas Aeruginosa
Author(s) -
Pham Thi Thu Ha,
Vũ Xuân Hòa,
Trinh Dinh Kha,
Nguyen Dac Dien,
Lương Duy Thành,
Nguyễn Quang Hưng,
Luong Van Luyen
Publication year - 2021
Publication title -
tạp chí khoa học đại học quốc gia hà nội: toán - lý (vnu journal of science: mathematics - physics)
Language(s) - English
Resource type - Journals
eISSN - 2615-9341
pISSN - 2588-1124
DOI - 10.25073/2588-1124/vnumap.4591
Subject(s) - bacillus subtilis , silver nitrate , nuclear chemistry , silver nanoparticle , fourier transform infrared spectroscopy , antibacterial activity , chemistry , pseudomonas aeruginosa , transmission electron microscopy , antimicrobial , nanoparticle , bacteria , materials science , nanotechnology , organic chemistry , chemical engineering , biology , engineering , genetics
In this study, the stable silver nanoparticles (AgNPs) were synthesized by reducing silver nitrate (AgNO3) using trisodium citrate (TSC). The product was characterized by Ultraviolet-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and X-ray diffraction analysis (XRD). UV-Vis spectrum showed a peak around 420 nm. TEM analysis revealed the homogeneity in the size of AgNPs (35-45 nm), well-dispersed quasi-spherical in water. The prepared AgNPs exhibited high antibacterial activity against Bacillus subtilis and Pseudomonas aeruginosa bacteria. The average zones of inhibition were 20 mm and 17 mm for Pseudomonas aeruginosa and Bacillus subtilis bacteria, respectively. The inhibition zone of AgNPs was also compared to the reference antibiotics drugs such as ampicillin and natamycin. This research exhibits an efficient and eco-friendly synthesis of silver nanoparticles with potent antimicrobial and antibacterial performance.