Open AccessEffect of carboplatin on malondialdehyde as a marker of lipoderoxidation in the submandibular gland of rats
Author(s) -
Evelin Bachmeier,
AUTHOR_ID,
ME Migueles Goitea,
JA Linares,
EG Ferreyra Mariani,
FM Wietz,
C. Dubersarsky,
Mabel Brunotto,
MA Mazzeo
Publication year - 2021
Publication title -
revista de la facultad de odontología
Language(s) - English
Resource type - Journals
eISSN - 2545-7594
pISSN - 0325-1071
DOI - 10.25014/revfacodont271.2021.31.2.9
Subject(s) - malondialdehyde , submandibular gland , superoxide dismutase , saline , medicine , endocrinology , carboplatin , chemistry , oxidative stress , chemotherapy , cisplatin
The objective of the present work was to evaluate the effect of carboplatin (Cp) on submandibular gland homogenate of Wistar rats through the determination of malondialdehyde levels, as the main end product of lipoperoxidation, in an experimental model.Sixteen three-month-old male Wistar rats were used, housed in individual cages, with controlled temperature and lighting and free diet. A completely randomized design was used and two experimental groups were established: 1) Control (C), administering an intraperitoneal dose of saline solution for one day, n: 8, 2) Animals treated with carboplatin (Cp) applying a dose i.p. of 100 mg / Kg of body weight for one day, n: 8. The animals were fasted for 24 hours and subsequently anesthetized. Then both submandibular glands were removed. Malondialdehyde levels were analyzed in submandibular gland homogenatein both experimental groups. Variations between the groups analyzed were evaluated using the Student's t test for paired samples, setting a p-value <0.05 for statistical significance. Project approved by CICUAL. Faculty of Medical Sciences (UNC).The group of Rats Co showed a concentration of 7.32± 0.48μmol / mg of gland. The Cp group had a concentration of 12.57 ± 0.71 μmol / mg of gland, expressing a significant decrease compared to the control group p <0.0006. Cp at the dose tested would cause a decrease in lipoperoxidation in the submandibular gland of rats. Possibly the glandular antioxidant battery would neutralize the oxidative stress of acinar cells. These results suggest a future evaluation of superoxide dismutase (SOD) activity and uric acid (UA) levels