Open AccessEffect of Synthetic Red Dye Orange Red and Natural Red Dye Alizarin on Biochemical and Hematological Parameters in Male Wistar Rats
Author(s) -
Ishfaq Khan,
Niamat Ali,
Mohd Murtaza
Publication year - 2019
Publication title -
international journal of pharmaceutical sciences and drug research
Language(s) - English
Resource type - Journals
ISSN - 0975-248X
DOI - 10.25004/ijpsdr.2019.110511
Subject(s) - alizarin red , orange (colour) , chemistry , distilled water , alizarin , biochemistry , chromatography , food science , staining , biology , nuclear chemistry , genetics
Nowadays synthetic food dyes are mostly preferred than natural plant derived dyes due to low cost and intense coloration. In this study hematological and biochemical parameters were determined in male wistar rats after 30 days treatment with synthetic red dye orange red and natural plant derived red dye alizarin. 25 male wistar rats were divided into 5 groups with 5 animals per group. Group I rats were taken as control treated with normal rat diet and distilled water. Group II and III rats (experimental) were oral gavaged with 50 mg and 150 mg/kg body weight of alizarin dye. Group IV and V rats (experimental) were gavaged with 50 mg and 150 mg/kg body weight of orange red dye. Treatment of group V rats with 150 mg/kg body weight of orange red dye produce significant changes in RBC, Hb, Hct, MCH, serum aminotransferase enzymes and serum protein fraction. In comparison to this in group IV rats a significant change was observed only in Hb, serum aminotransferase enzymes and serum protein fraction when compared with control (group I) rats. However in group II and III alizarin treated rats no significant change was observed in different biochemical and hematological parameters relative to their respective control. In conclusion synthetic orange red dye proved to be more toxic than natural plant derived red dye alizarin.