Open AccessAcute Toxicity Studies of Petroleum Ether, Methanol and Aqueous Extracts of Nigella sativa
Author(s) -
Chandrashekaran Girish,
Y. V. R. Reddy
Publication year - 2017
Publication title -
international journal of pharmaceutical sciences and drug research
Language(s) - English
Resource type - Journals
ISSN - 0975-248X
DOI - 10.25004/ijpsdr.2017.090210
Subject(s) - nigella sativa , petroleum ether , acute toxicity , toxicity , oral administration , traditional medicine , methanol , median lethal dose , pharmacology , chemistry , toxicology , medicine , chromatography , biology , organic chemistry , extraction (chemistry)
The purpose of the study was to test the acute oral toxicity of the different extracts of the plant Nigella sativa. Acute toxicity of petroleum ether, methanol and aqueous extracts of Nigella sativa was evaluated in Swiss mice. The acute toxicity studies were carried out based on OECD guidelines 423. The animals were administered orally with a single dose of 100, 250, 500, 750, 1000, 2000 mg/kg body weight of each extract. Signs of toxicity and mortality were noted after 1, 4 and 24h of administration of the extract for about 14 days. The highest dose administered (2000 mg/kg body weight) do not produce mortality or changes in general behaviour of the test animals. These results indicate the safety of the oral administration of petroleum ether, methanol and aqueous extracts of Nigella sativa.