Open AccessAntibacterial and Antioxidant Activities of Ethanolic Leaves Extracts of Dissotis multiflora triana (Melastomataceae)
Author(s) -
Afagnigni Alian Désiré,
Nyegue Maximilienne Ascension,
Ndoye Foe Florentine,
Voundi Steve,
Fonkoua Marie Christine,
FrançoisXavier Etoa
Publication year - 2016
Publication title -
international journal of pharmaceutical sciences and drug research
Language(s) - English
Resource type - Journals
ISSN - 0975-248X
DOI - 10.25004/ijpsdr.2016.080108
Subject(s) - phytochemical , dpph , ascorbic acid , abts , enterobacter cloacae , chemistry , shigella flexneri , food science , bacillus cereus , proteus mirabilis , agar diffusion test , antibacterial activity , traditional medicine , broth microdilution , minimum inhibitory concentration , gallic acid , klebsiella pneumoniae , antioxidant , biology , antimicrobial , escherichia coli , bacteria , biochemistry , medicine , gene , genetics , organic chemistry
Dissotis multiflora leaves are commonly used in the Cameroon traditional medicine to heal infectious diseases without solid scientific basis. This study aimed to investigate the phytochemical screening, in vitro antibacterial activity, phenol content and antioxidant activity of its ethanolic leaves extracts. Antibacterial test was assessed by agar well diffusion and microdilution methods against seven clinical isolates species namely Enterobacter cloacae, Klebsiella pneumoniae, Escherichia coli, Shigella flexneri, Proteus mirabilis, Salmonella typhi and Bacillus cereus. Total phenol content was determined by the Folin-Ciocalteu test while antioxidant activity was evaluated by DPPH, FRAP and ABTS methods. The phytochemical screening revealed the presence of phenols, tannins, alkaloids, saponins, anthraquinons, steroids and flavonoids. Extract inhibited the growth of all species tested with inhibition zone diameter ranged from 10.33 ± 1.24 mm (Klebsiella pneumoniae) to 28 ± 2.94 mm (Shigella flexneri) and MIC varying between 97.65µg/ml (Salmonella typhi) and 781µg/ml (Bacillus cereus and Enterobacter cloacae). Total phenol content was found to be 813.68 ± 1.27 mg ascorbic acid equivalent/g of dry material. The scavenging kinetics of DPPH by Dissotis multiflora extracts shows a steady state at 60 minutes while EC50 was 0.7 × 103 g/mol in DPPH. FRAP assay shows a reducing power of 7 ± 0.35 mg ascorbic acid equivalent/g of dry material. ABTS assay displayed an inhibition percentage of 93.269% at 4.54µg/ml. These results justify the use of Dissotis multiflora in traditional medicine to heal diarrhea and can be use as potential biomolecules reservoir.