Open AccessSpectrophotometric Estimation of Losartan Potassium with Methylene Blue by Ion-Pair Extraction Method
Author(s) -
Asmaa Ghanim Dawood,
Lazeeza Sattar Omer
Publication year - 2020
Publication title -
iraqi journal of science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.152
H-Index - 4
eISSN - 2312-1637
pISSN - 0067-2904
DOI - 10.24996/ijs.2020.61.12.1
Subject(s) - losartan potassium , absorbance , methylene blue , detection limit , molar absorptivity , extraction (chemistry) , chemistry , chromatography , losartan , aqueous solution , dichloromethane , potassium ferricyanide , potassium , analytical chemistry (journal) , inorganic chemistry , angiotensin ii , solvent , organic chemistry , biochemistry , physics , receptor , photocatalysis , optics , catalysis
A selective and sensitive spectrophotometric extraction method was established and used to estimate antihypertensive drug, losartan potassium. The method is based on the formation of blue ion pair of the anionic drug, losartan, and the cationic dye, methylene blue, at adjusted pH 6.5 in aqueous solutions, followed by quantitative extraction to dichloromethane;.The observed maximum absorbance was at π 654.9 nm. With 4.53321 x 105 M-1 cm-1 molar absorptivity, Beer's law was obeyed within a concentration range of 0.03-1.5 μg / ml. The limit of detection and the limit of quantification were 0.01μg / ml and 0.03μg / ml, respectively. The method's precision was estimated by a relative standard deviation (0.725%-1.64%), and the accuracy was validated at a recovery range (98.48-100.3%). The proposed method was successfully used for estimating losartan in the pharmaceutical formulation and in the urine sample.