Open AccessFlow Sitometri ile Bazı Ispanak Aksesyonlarının Çekirdek DNA İçeriklerinin Belirlenmesi
Author(s) -
Murat Devecí,
Özcan Yavaş,
Nihan Şahin,
Metin Tuna
Publication year - 2018
Publication title -
türk tarım - gıda bilim ve teknoloji dergisi
Language(s) - English
Resource type - Journals
ISSN - 2148-127X
DOI - 10.24925/turjaf.v6i2.239-246.1718
Subject(s) - spinach , biology , ploidy , nuclear dna , spinacia , chromosome , horticulture , accession number (library science) , chromosome number , botany , genetics , mitochondrial dna , karyotype , gene , biochemistry , chloroplast , genbank
The aim of this research is to determine ploidy levels of 53 spinach (Spinacia oleracea L.) accessions obtained from abroad sources by using flow cytometry. According to the results of the statistical analysis, the differences among DNA content of accessions were statistically important. The average DNA content of spinach accessions used in the study ranged between 2.225 pg/2C (Esfenaj accession) and 2.059 pg/2C (Matador and Godir accessions). The mean nuclear DNA of accessions shown to be relatively stable due to low standard deviation (0.003-0.096). Based on the mitotic chromosome analyses of the some plants with different DNA contents, mitotic chromosome number of the all plants analyzed in the study were determined as 2n=12. These results indicate that all the accessions used in the study are diploid although their DNA content is significantly vary. According to the clustering analysis of the 2C DNA contents results, it was observed that eight sub clusters were formed under two main clusters. The clustering of axioms was performed in a similar manner to the multiple comparison test. As a result, the data will save time and labor convenience in a further breeding studies deal with the same 53 spinach accessions of our study.