Open AccessUp-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro
Author(s) -
Yimin Zhu,
Yimin Zhu,
Fuying Xu,
Fuying Xu
Publication year - 2017
Publication title -
chinese medical sciences journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.215
H-Index - 21
ISSN - 1001-9294
DOI - 10.24920/j1001-9242.2007.006
Subject(s) - apoptosis , flow cytometry , medicine , in vitro , small hairpin rna , microbiology and biotechnology , cell culture , cancer research , cell , gene expression , gene , immunology , biology , gene knockdown , genetics
Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.
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